Identification of New Natural CphA Metallo-β-Lactamases CphA4 and CphA5 in Aeromonas veronii and Aeromonas hydrophila Isolates from Municipal Sewage in Central Italy.
نویسندگان
چکیده
Two new natural CphA metallo-β-lactamases, the CphA4 and CphA5 enzymes, were identified in water samples from municipal sewage in central Italy. Compared to CphA, the CphA4 and CphA5 enzymes showed numerous point mutations. These enzymes have a narrow spectrum of substrates focused on carbapenems only. CphA5 showed kcat values about 40-, 12-, and 97-fold higher than those observed for CphA4 versus imipenem, ertapenem, and biapenem, respectively.
منابع مشابه
Extended-spectrum β-lactamase and carbapenemase-producing Aeromonas species in wild animals from Portugal.
AeromonAs are Gram-negative, facultative-anaerobic, non-sporeforming, glucose-fermenting, oxidaseand catalase-positive rods (Martin-Carnahan and Joseph 2005). Apart from fish, which are widely reported hosts for Aeromonads, insects, crustaceans, reptiles, birds and mammals were also found to harbour Aeromonas species, both in healthy and disease state (Pearson and others 2000, Turutoglu and oth...
متن کاملOccurrence and Variety of β-Lactamase Genes among Aeromonas spp. Isolated from Urban Wastewater Treatment Plant
Members of the genus Aeromonas that commonly occur in various aquatic ecosystems are taken into account as vectors spreading antibiotic resistance genes (ARGs) in the environment. In our study strains of Aeromonas spp. (n = 104) not susceptible to ampicillin were isolated from municipal sewage of different levels of purification - raw sewage, activated sludge and treated wastewater. The crucial...
متن کاملClinical Implications of Species Identification in Monomicrobial Aeromonas Bacteremia
BACKGROUND Advances in Aeromonas taxonomy have led to the reclassification of aeromonads. Hereon, we aimed to re-evaluate the characteristics of Aeromonas bacteremia, including those of a novel species, Aeromonas dhakensis. METHODOLOGY/PRINCIPAL FINDINGS A retrospective study of monomicrobial Aeromonas bacteremia at a medical center in southern Taiwan from 2004-2011 was conducted. Species ide...
متن کاملConvenient test using a combination of chelating agents for detection of metallo-beta-lactamases in the clinical laboratory.
Although transmissible metallo-beta-lactamases (MBLs) are a serious threat to beta-lactam antibiotic therapy, the CLSI currently does not recommend testing methods for the detection of MBLs. The aim of this study was to evaluate the capability of double-disk tests (DDTs) by using disks containing a combination of the chelators 2-mercaptopropionic acid (MPA) and Tris-EDTA (TE) to detect MBLs. Si...
متن کاملCompetitive Inhibitors of the CphA Metallo- -Lactamase from Aeromonas hydrophila
Competitive Inhibitors of the CphA Metallo-Lactamase from Aeromonas hydrophila L. E. Horsfall,† G. Garau,† B. M. R. Liénard, O. Dideberg, C. J. Schofield, J. M. Frère, and M. Galleni* Centre d’Ingéniérie des Protéines, Université de Liège, Allée de 6 Aout B6, Sart-Tilman, Liège, Belgium; Institut de Biologie Structurale Jean-Pierre Ebel (CNRS/CEA/UJF), 41 Rue J. Horowitz, Grenoble 38100, France...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 59 8 شماره
صفحات -
تاریخ انتشار 2015